No effect of probiotics from the Lactobacillus and Bifidobacterium genera in the control of bacterial plaque in crab-eating fox Cerdocyon thous kept in human care

Abstract

This study evaluated the effects of synergistic probiotic therapy on the formation of dental bacterial plaque in crab-eating foxes Cerdocyon thous in human care. Six crab-eating foxes with periodontal disease grades I and II were randomly divided into two groups of three animals. A total of 66 teeth were assessed per group. Group 1 received probiotics in powder form sprayed onto food once a day for 60 days. Group 2 received no probiotics but similar feeding as Group 1 subjects. The probiotic contained 1 billion organisms of Lactobacillus reuteri, Lactobacillus salivarius, Lactobacillus brevis and Bifidobacterium bifidum. Maxillary teeth were stained with 2% eosin and photographed before treatment and 60 days after cleaning. The images of maxillary and mandibular teeth were inspected using Photoshop software to obtain each tooth’s total two-dimensional area and the bacterial plaque or dental calculus area. There was no significant difference in the global percentage of bacterial plaque and dental calculus between groups before and after treatment. The synergistic use of probiotics from the Lactobacillus and Bifidobacterium genera did not result in a statistically significant difference between groups. There were signs of probiotic adherence to some teeth.