TY - JOUR AU - Tremblay-Gratton, Anne AU - Boussin, Jean-Christophe AU - Bennachi, Aymen AU - Tamigneaux, Eric AU - Vandenberg, Grant AU - Le François, Nathalie Rose PY - 2017/04/30 Y2 - 2024/03/29 TI - Implementation of sulphur denitrification in a large-scale fully recirculated cold-SW aquarium: A sustainability practice JF - Journal of Zoo and Aquarium Research JA - JZAR VL - 5 IS - 2 SE - Evidence Based Practice DO - 10.19227/jzar.v5i2.288 UR - https://jzar.org/jzar/article/view/288 SP - 104-108 AB - <p align="JUSTIFY" dir="LTR"><span style="font-size: xx-small;">Autotrophic sulphur-based denitrification (ASD) was evaluated to control nitrate accumulation in the marine aquatic exhibit of the Biodôme de Montréal (Montréal, QC, Canada). Different substrates (two sulphur and two calcareous sources were tested), flow-rates and configurations (homogenous and low to high stratification levels) were evaluated that successively improved nitrate removal rates (g N-NO3- </span><span style="font-size: xx-small;">· </span><span style="font-size: xx-small;">day-1) and/or start-up time. Despite pronounced suboptimal conditions for anaerobic denitrifying bacteria, Thiobacillus denitrificans i.e. saline (24 PSU), cold (5-10<sup>o</sup>C) and aerated waters, our R&amp;D efforts lead to the development of an efficient, simple, custom-made ASD unit adapted to a priori unfavourable conditions. The Rocky Shore exhibit (25 m<sup>3</sup>) housing the most sensitive marine aquatic species of our live collections (mostly invertebrates) previously operated at ? 50 mg N-NO3- </span><span style="font-size: xx-small;">· </span><span style="font-size: xx-small;"> L-1 is now at ~ 20 mg N-NO3- </span><span style="font-size: xx-small;">· </span><span style="font-size: xx-small;">L-1. Considering the significant economic and environmental gains achieved (e.g. sustainability) following the implementation of this technology at a small-scale on the Rocky Shore exhibit (100 kg of sulphur), a 10-fold upscaling (1000 kg of sulphur) ASD unit to be connected to the La Baie exhibit (2500 m<sup>3) </sup>is planned.</span></p> ER -